Lumbrokinase

Lumbrokinase is a group of fibrinolytic enzymes derived primarily from the earth‑worm Lumbricus rubellus (and related species). In supplement form it is used mainly to support normal blood‑clot breakdown (fibrinolysis) and to promote healthy circulatory function.

• Composition: Lumbrokinase is not a single molecule but a mixture of serine proteases (e.g., lumbrokinase 1, 2, 3) with molecular weights ranging from 25 kDa to 30 kDa.
• Structure: Each enzyme is a polypeptide of ~250–300 amino acids, containing the classic catalytic triad (His‑57, Asp‑102, Ser‑195) typical of the trypsin family.
• Molecular Formula: The overall “molecular formula” varies by isoform, but an average composition can be approximated as C₁₁₅₀H₁₈₅₀N₃₅₀O₃₇₀S₁₀, reflecting a typical protein.
• Nomenclature: No single IUPAC name exists; the enzymes are classified as “serine proteases (EC 3.4.21)”.
• Key Structural Features: Key structural features include a conserved “catalytic pocket” that binds the Arg‑Gly‑Asp sequence of fibrin, and a glycosylation pattern that improves stability in the gastrointestinal tract.

• Source: Commercial Lumbrokinase is extracted primarily from the dried bodies of Lumbricus species (most commonly Lumbricus rubellus and Eisenia fetida) harvested from controlled, pesticide‑free farms.
• Extraction Process: The process involves aqueous extraction, filtration, and purification by ion-exchange chromatography to isolate the active protein fractions.
• Recombinant Production: Some manufacturers use recombinant expression in E. coli or Pichia pastoris to produce a more defined enzyme mixture, which can improve batch‑to‑batch consistency.
• Quality Control: Quality‑control standards include activity assays (FU per milligram), absence of heavy metals, and microbial limits according to USP <111> and GMP guidelines.
• Product Selection: For optimal safety, look for products that disclose the source species, extraction method, and provide third‑party testing for potency and contaminants.